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. 2021 Feb 12;12:980. doi: 10.1038/s41467-021-21210-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Number of IS150 copies in genomes of the ancestor (Anc) and six evolved clones: 1158C (2000 generations), 9282B (20,000 generations), 10450 (30,000 generations), and 11008, 11009 and 11010 (40,000 generations). b Frequencies of IS150-mediated transposition (black) and recombination (white) events, measured using the reporter plasmid pFDX2339. c Proportion of transposition (black) and recombination (white) events, expressed relative to the total number of events (including both transposition and recombination). Error bars show the standard deviation based on three technical replicates for each of three biological replicates, except for the ancestor which had three technical replicates for each of five biological replicates and 1158C and 11009, which had three technical replicates for each of two biological replicates. Source data are provided as a Source Data file.