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. 2021 Feb 12;10(2):15. doi: 10.1038/s41389-021-00304-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Wound healing assay of shALOXE3-U87 and shCont-U87 cells was determined at 0 and 24 h after the wound was created. The right panel is the percentage of the wound closed at 24 h (n = 6). B Transwell assay of shALOXE3-U87 and shCont-U87 cells. The right panel is the quantification of the number of migrated cells (n = 6). C, D Levels of (C) 12-HETE and (D) 12-KETE in conditional medium (CM) harvested from shALOXE3-U87 and shCont-U87 cells treated with ML355 or DMSO as control (n = 6). E, F U87 cells were incubated with CM harvested from shALOXE3-U87 and shCont-U87 cells treated with ML355 or DMSO as vehicle control, and followed with (E) wound healing assay and (F) transwell assay (n = 6). G, H U87 cells were treated with 12-HETE or Vehicle control, and followed with (G) wound healing assay and (H) transwell assay (n = 6). All data are represented as the mean ± s.e.m. *p < 0.05 (Student’s t-test).