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Components 5 × artificial urine, dissolved in 1 L of milliQ water and autoclaved.
| Components | Mass (g) |
|---|---|
| Anhydrous sodium sulphate | 11.50 |
| Magnesium chloride hexahydrate | 3.25 |
| Sodium chloride | 23.00 |
| Tri-sodium citrate | 3.25 |
| Sodium oxalate | 0.10 |
| Potassium di-hydrogen orthophosphate | 14.00 |
| Potassium chloride | 8.00 |
| Ammonium chloride | 5.00 |
| Gelatine | 25.00 |
| Tryptone soya broth | 5.00 |
The pH was adjusted to pH = 5.7 by the addition of sodium hydroxide (all components were purchased from Merck, Germany).
