Table 1.
Kinetic parameters for AM3 binding to the immobilized liposomes.a
| DOPC | DOPC/Chol (9:1) | DOPC/Chol (7:3) | SM | SM/Chol (9:1) | SM/Chol (7:3) | |
|---|---|---|---|---|---|---|
| ka1 ( × 103/Ms) | 1.49 | 1.90 | 1.39 | 0.770 | 2.42 | 1.72 |
| kd1 ( × 10−2/s) | 9.54 | 1.20 | 0.582 | 10.6 | 7.76 | 9.65 |
| ka2 ( × 10−2/s) | 1.53 | 1.23 | 1.31 | 1.56 | 1.23 | 1.50 |
| kd2 ( × 10−5/s) | 156 | 8.33 | 7.44 | 72.4 | 25.5 | 22.9 |
| KD1 ( × 10−6/M) | 64.0 | 6.32 | 4.19 | 138 | 32.1 | 56.1 |
| KD2 ( × 10−3) | 102 | 6.77 | 5.68 | 46.4 | 20.7 | 15.3 |
| KD ( × 10−8/M) | 652 | 4.27 | 2.38 | 640 | 66.4 | 85.7 |
a
The data were obtained using two state reaction model. AM3 concentration was 50 μM. Standard errors are within 5% (n = 3).