A: Spatial distribution of m/z 401.343, annotated as 7-ketocholesterol, in purple, and m/z 369.350, identified as cholesteryl ion, in green. 7-Ketocholesterol shows a spatial distribution distinct from that of other lipids in our dataset. B: Combined MALDI-MSI image of spatial distributions of a CE in purple (m/z 671.580), SM in green (m/z 701.571), and a TG in white (m/z 929.767) for three tissue sections. CE is found throughout the tissue section, whereas SM and TG show a more localized spatial pattern. Scale bars are 1 mm.