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. 2021 Jan 27;24(2):102093. doi: 10.1016/j.isci.2021.102093

Figure 2.

Figure 2

IL-27Rα expression is associated with an increased frequency of TIGIT expression on memory CD4+ T cells during sepsis

The gating strategy for PD-1 and TIGIT is shown inFigure S2.

(A) Representative flow cytometric plots showing PD-1 and TIGIT expression on CD44hi memory CD4+ T cells lacking (black) or expressing (blue) IL-27Rα in wild-type mice on days 1–4 after sham surgery (“sham”) or CLP.

(B) The frequency of TIGIT+ expressing IL-27Rα− (black) and IL-27Rα+ (blue) CD44hi memory CD4+ T cells in sham and CLP wild-type mice on days 1–4 after surgery.

(C) The frequency of PD-1+ expressing IL-27Rα− (black) and IL-27Rα+ (blue) CD44hi memory CD4+ T cells in sham and CLP wild-type mice on days 1–4 after surgery.

(D) Representative flow cytometric plots showing TIGIT and PD-1 expression on CD44hi memory CD8+ T cells lacking (black) or expressing (blue) IL-27Rα in sham mice and CLP wild-type mice on days 1–4 after surgery.

(E) The frequency of TIGIT+ expressing IL-27Rα− (black) and IL-27Rα+ (blue) CD44hi memory CD8+ T cells in sham and CLP wild-type mice on days 1–4 after surgery.

(F) The frequency of PD-1+ expressing IL-27Rα− (black) and IL-27Rα+ (blue) CD44hi memory CD8+ T cells in sham and CLP wild-type mice on days 1–4 after surgery. Data were pooled from three independent experiments, with n = 7–17 mice per group. ∗∗p < 0.01. Error bars represent the mean ± the standard deviation.