Figure 5.

Il27ra^−/− septic mice have a reduced frequency of TIGIT⁺ memory CD4⁺ T cells

Wild-type and Il27ra^−/− mice underwent sham surgery (“sham”) or CLP surgery. On days 1 and 2 following surgery, splenocytes were collected and used for flow cytometric analysis.

(A) Representative flow cytometric plots (left) and summary graphs (right) showing TIGIT expression (x axis) in CD44^hi memory CD4⁺ T cells of wild-type (top) and Il27ra^−/− (bottom) mice.

(B) Representative flow cytometric plots (left) and summary graphs (right) showing PD-1 expression (x axis) in CD44^hi memory CD4⁺ T cells of wild-type (top) and Il27ra^−/− (bottom) mice.

(C) Representative flow cytometric plots (left) showing TIGIT expression (x axis) in CD44^hi memory CD8⁺ T cells of wild-type (top) and Il27ra^−/− (bottom) mice.

(D) Representative flow cytometric plots (left) and summary graphs (right) showing PD-1 expression (x axis) in CD44^hi memory CD8⁺ T cells of wild-type (top) and Il27ra^−/− (bottom) mice. Data were pooled from two independent experiments, with n = 7–11 mice per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Error bars represent the mean ± the standard deviation.