Figure 7.

Memory CD4⁺ T cells in Il27ra^−/– mice exhibit impaired production of IFN-γ at baseline and during sepsis

CLP and sham surgery (“sham”) were performed on wild-type and Il27ra^−/− mice. One day after surgery, splenocytes were harvested for stimulation with PMA/Ionomycin or incubated without stimulation. Following incubation, samples were stained for IFN-γ and TNF-α and assessed by flow cytometry.

(A) Representative flow cytometric plots of IFN-γ (y axis) and TNF-α (x axis) production by the CD44^hi memory CD4⁺ T cells of wild-type sham (left) and wild-type CLP mice (right) one day following surgery. The top row shows unstimulated controls, and bottom row shows stimulated samples.

(B) The frequency of IFN-γ producing CD44^hi memory CD4⁺ T cells of wild-type (black) and Il27ra^−/− (blue) mice following surgery.

(C) The frequency of TNF-α-producing CD44^hi memory CD4⁺ T cells of wild-type (black) and Il27ra^−/− (blue) mice following surgery.

(D) The frequency of IFN-γ and TNF-α co-producing CD44^hi memory CD4⁺ T cells of wild-type (black) and Il27ra^−/− (blue) mice following surgery.

(E) Representative flow cytometric plots of IFN-γ (y axis) and TNF-α (x axis) production by the CD44^hi memory CD8⁺ T cells of wild-type sham (left) and wild-type CLP mice (right) one day following surgery. The top row shows unstimulated controls, and bottom row shows stimulated samples.

(F) The frequency of IFN-γ-producing CD44^hi memory CD8⁺ T cells of wild-type (black) and Il27ra^−/− (blue) mice following surgery.

(G) The frequency of TNF-α-producing CD44^hi memory CD8⁺ T cells of wild-type (black) and Il27ra^−/− (blue) mice following surgery.

(H) The frequency of IFN-γ and TNF-α co-producing CD44^hi memory CD8⁺ T cells of wild-type (black) and Il27ra^−/− (blue) mice following surgery. Data are representative of one experiment with n = 4–5 mice per group. ∗p < 0.05, ∗∗p < 0.01. Error bars represent the mean ± the standard deviation.