Figure 3.

Effect of Orco CBS mutation on the odor response in OrX/Orco expressing OSNs using vinegar and grape juice as stimulus. (a) Time course of Ca²⁺ fluorescence responses obtained in OrX/OrcoWT OSNs and OrX/OrcoK339N OSNs upon OR stimulation with 10^–2 concentration of vinegar (n = 5 for OrcoWT, n = 5 for OrcoK339N). (b) Concentration response relationship for OrcoWT and OrcoK339N OSNs (n = 5). (c) Maximum Ca²⁺ fluorescence response (Max ∆F/F₀ (%)) obtained by subtracting the base level at 50 s from the maximum Ca²⁺ fluorescence at 10^–1 dilution. (d) Time course of Ca²⁺ fluorescence responses obtained in OrcoWT and OrcoK339N flies upon OR stimulation with pure grape juice (n = 5 for OrcoWT, n = 5 for OrcoK339N). (e) Concentration response relationship for OrcoWT and OrcoK339N OSNs (n = 5). (f) Maximum Ca²⁺ fluorescence response (Max ∆F/F₀ (%)) obtained by subtracting the base level at 50 s from the maximum Ca²⁺ fluorescence obtained for pure grape juice. n = number of antennae. Two-tailed unpaired t- test, *P < 0.05, ns = not significant. Data given as mean ± SEM.