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. 2021 Feb 12;11:3747. doi: 10.1038/s41598-021-83296-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Orco CBS mutation on the odor odor-guided behaviour. (a) Schematic diagram of wind tunnel bioassay. Odor guided behavior is monitored as landing of Drosophila melanogaster on filter paper containing balsamic vinegar (10^–2 dilution) (left) or pure grape juice (right). (b, c) Landing percentage (L %) of OrX/Orco flies in OrcoWT, OrcoWT-R, OrcoK339N, and Orco^-/- condition with vinegar (10^–2 dilution) (a) and pure grape juice (b). (d, e) Landing percentage (L %) of Or22a/Orco flies in OrcoWT-R, OrcoK339N, and Orco^-/- condition with vinegar (10^–2 dilution) (d) and grape juice (e). For (d) not all the values were different from each other. n = 15 replicates, each replicate consists of 15 mated female flies. Kruskal–Wallis test with Dunn’s Multiple Comparison Test, *P < 0.05, **P < 0.01, ***P < 0.001, otherwise not significant. Data given as mean ± SEM.