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. 2021 Feb 9;2(1):100334. doi: 10.1016/j.xpro.2021.100334

Figure 2.

Figure 2

Visualized timeline of hiPSC-CM expansion

(A) Timeline diagram displaying the steps required for expansion and passaging of hiPSC-CMs.

(B) Time-lapse images of cell morphology at the indicated passage numbers and/or culture days. Left: Images of cell confluency on day of passaging for P0-5. Right: Daily consecutive images of P1 hiPSC-CMs demonstrating cell seeding density and growth speed between P1 and P2. Replating at day 15, start of CHIR9021 (CHIR) treatment at day 16, cell cluster formation at day 14–19 is observed the days after and a nearly confluent monolayer before passaging at D18–20. Scale bar, 200 μm. D, day; P, passage.