Table 1.

Key culture format cultivation information

steps	Culture format	Culture formats #	Start amount of cells (104/cm2)	Total amount of cells after each step	Volume medium/format	Timing (days)
hiPSC culturing	6 wells plate	1 well	~2	~300	2 mL per wells	4
Cardiac differentiation	6 wells plate	1 well	~30	~700	3 mL per wells	11
Passage 1	T75 flask	1×	~2.5	~2,100	7 mL per T75 flask	~4
Passage 2	T175 flask	3×	~4	~7,000	21 mL per T175 flask	~5
Passage 3	T175 flask	8×	~5	~20,000	21 mL per T175 flask	~6
Passage 4	T175 flask	14×	~8	~35,000	21 mL per T175 flask	~7
Passage 5	T175 flask	20×	~10	~5,000	21 mL per T175 flask	~8