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. 2021 Jan 20;47:101170. doi: 10.1016/j.molmet.2021.101170

Figure 3.

Figure 3

SELENOI deficiency decreased T cell proliferation. (A) WT and SELENOI KO T cells or (B) No DOX control and DOX-induced SELENOI KD T cells were visualized after 18 h of activation for density of proliferating clusters. KO and KD T cells exhibited fewer clusters. Scale bar = 125 mm. (C–E) WT and SELENOI KO T cells were loaded with CSFE and its decreasing fluorescent intensity with each round of cell division was measured by flow cytometry, which allowed cell numbers in each cell division group to be determined. After 3 d of activation, T cells were analyzed for the Proliferation Index as described in the Methods section. (F–H) CSFE stained No DOX controls or DOX-induced SELENOI KD T cells were analyzed for proliferation after 3 d of activation. Means of replicates (N = 4) were compared using Student's t test and expressed as mean ± SEM with ∗p < 0.05.