Figure 4.

In vivo antigen challenge demonstrated decreased proliferation in SELENOI KO T cells. Spleen cells from LCMV gp33-41 vaccinated mice were analyzed by flow cytometry using the H-2D^b-KAVYNFATM-APC tetramer. (A–C) The gating strategy is shown in which single cells were analyzed for lymphocytes excluding debris that were gated for live cells that were then analyzed for CD16/32⁻CD8⁺ non-phagocytic T cells. (D) The latter cells were analyzed for percent antigen experienced CD44⁺tetramer⁺ T cells. Representative dot plots of samples from WT and KO mice injected with adjuvant alone or adjuvant plus LCMV gp33-41 peptide are shown. (E) The results showed that KO mice generated lower levels of antigen-specific T cells compared to WT controls. Means of replicates (n = 4 mice/group) were compared using Student's t test and expressed as mean ± SEM with ∗p < 0.05.