Figure 6.

Dorsomorphin treatment inhibited T cell proliferation. Activated WT T cells were treated with different doses of dorsomorphin during CSFE assays. (A) Images of cultured cells at 18 h post-TCR-stimulation with clusters of proliferating cells decreasing with increasing doses of dorsomorphin. Scale bar = 125 mm. (B) Flow cytometry data used to calculate the Proliferation Index. (C) CSFE assays using different concentrations of the AMPK inhibitor, dorsomorphin, decreased the Proliferation Index from the data shown in Figure S5. (D) Western blot analysis showed that 0.75 mM of dorsomorphin treatment led to decreased phosphorylation of AMPK after 5 h of TCR stimulation similar to SELENOI KO. Means of replicates (n = 3) were compared using Student's t test and means in panel I (n = 4) were analyzed using one-way ANOVA with Tukey's post-test and expressed as mean ± SEM with ∗p < 0.05.