Figure 8.

The GPI anchor synthesis/attachment pathway was disrupted in SELENOI KO T cells. (A) Real-time PCR showed that relative mRNA levels for GPAA1 decreased in SELENOI KO T cells compared to WT controls after 18 h of activation. (B–C) Representative Western blotting and densitometry analysis of GPAA1 protein levels from 3 replicate experiments showed lower levels in SELENOI KO T cells after 18 h of activation, and β-actin was used as a loading control. (D) Flow cytometry was used to measure cell surface levels of a panel of 8 GPI-anchored proteins prior to and 1–3 days after TCR stimulation. CD45.2 was included as a non-GPI-anchored protein control. Means of replicates (n = 3) were compared using Student's t test and expressed as mean + SEM with ∗p < 0.05.