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. Author manuscript; available in PMC: 2021 Feb 13.
Published in final edited form as: Clin Sci (Lond). 2020 Jul 17;134(13):1763–1774. doi: 10.1042/CS20200184

Figure 5. Deletion of Ctcf affects somatic and kidney growth and leads to severe kidney abnormalities.

Figure 5.

A. Kidneys from Ctcf-cKO mice are small, pale, and with irregular surfaces. B. Body weights of Ctcf-cKO mice (n=12) were significantly lower than in control animals (n=7). C. The kidney/body weight ratio was markedly decreased in Ctcf-cKO mice. D. A tiled image of H&E staining reveals disorganized kidney structure, lack of cortical-medullary junctions, and dilated tubules in kidneys from Ctcf-cKO mice (bottom). E. Left, Masson’s trichrome shows interstitial fibrosis (blue) with cortical depressions (arrow) in Ctcf-cKO kidneys. Also, peri and intraglomerular fibrosis are observed. Middle, PAS staining indicates intraluminal casts in dilated tubules (black arrows) and sclerotic glomeruli with fibrocellular crescent formation (yellow arrow) surrounded by disorganized packed cells. Right, αSMA immunostaining shows an increased expression of αSMA in the interstitium, around and within glomeruli. Quantitative data are presented as mean ± SD. *p<0.05, ***p<0.001, by unpaired, 2-sided Student’s t-test. Black squares, males; red dots, females.