Table 1.
Primers | Sequences (53) | Target | PCR conditions | Amplicon size (bp) | |||
---|---|---|---|---|---|---|---|
Forward | Reverse | Denaturation | Annealing | Extension | |||
Universal | GACGGVGCRACTACATGGT | GTCCAATTCATNCCTGGTG | VP6 | 94°C/60s | 55°C/60s | 72°C/60s | 380 |
G typing | ATGTATGGTATTGAATATACCAC | AACTTGCCACCATTTTTTCC | VP7 | 94°C/60s | 52°C/60s | 72°C/60s | 881 |
CAAGTACTCAAATCAATGATGG | G1 | 94°C/60s | 42°C/120s | 72°C/60s | 620 | ||
CAATGATATTAACACATTTTCTGTG | G2 | 522 | |||||
ACGAACTCAACACGAGARG | G3 | 682 | |||||
CGTTTCTGGTGAGGAGTTG | G4 | 452 | |||||
GTCACACCATTTGTAAATTCG | G8 | 754 | |||||
CTTGATGTGACTAYAAATAC | G9 | 177 | |||||
CCGATGGACGTAACGTTGTA | G12 | 266 | |||||
P typing | TATGCTCCAGTNAATTGG | ATTGCATTTCTTTCCATAATG | VP4 | 94°C/60s | 50°C/60s | 72°C/60s | 663 |
CTATTGTTAGAGGTTAGAGTC | P4 | 94°C/60s | 45°C/120s | 72°C/60s | 362 | ||
TGTTGATTAGTTGGATTCAA | P6 | 146 | |||||
TCTACTTGGATAACGTGC | P8 | 224 |