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. 2020 Winter;13(Suppl1):S128–S133.

Table 1.

Used primers and PCR conditions in this study

Primers Sequences (53) Target PCR conditions Amplicon size (bp)
Forward Reverse Denaturation Annealing Extension
Universal GACGGVGCRACTACATGGT GTCCAATTCATNCCTGGTG VP6 94°C/60s 55°C/60s 72°C/60s 380
G typing ATGTATGGTATTGAATATACCAC AACTTGCCACCATTTTTTCC VP7 94°C/60s 52°C/60s 72°C/60s 881
CAAGTACTCAAATCAATGATGG G1 94°C/60s 42°C/120s 72°C/60s 620
CAATGATATTAACACATTTTCTGTG G2 522
ACGAACTCAACACGAGARG G3 682
CGTTTCTGGTGAGGAGTTG G4 452
GTCACACCATTTGTAAATTCG G8 754
CTTGATGTGACTAYAAATAC G9 177
CCGATGGACGTAACGTTGTA G12 266
P typing TATGCTCCAGTNAATTGG ATTGCATTTCTTTCCATAATG VP4 94°C/60s 50°C/60s 72°C/60s 663
CTATTGTTAGAGGTTAGAGTC P4 94°C/60s 45°C/120s 72°C/60s 362
TGTTGATTAGTTGGATTCAA P6 146
TCTACTTGGATAACGTGC P8 224