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. Author manuscript; available in PMC: 2021 Feb 13.
Published in final edited form as: Nat Immunol. 2020 May 18;21(6):626–635. doi: 10.1038/s41590-020-0681-x

Fig. 6 |. Deletion of IL-18 and NLRP6 rescues elevated IL-18 levels in colon tissues of Cyld−/− mice ex vivo.

Fig. 6 |

a, mRNA from colonic mucosa of age- and sex-matched C. rodentium-infected wild-type, Cyld−/−, Il18−/− and Cyld−/−Il18−/− mice (n=8 mice per group) was isolated, and the expression of Il18 was quantified by real-time PCR (NSP=0.3390). b, Colon tissues from C. rodentium–infected wild-type, Cyld−/−, Il18−/− and Cyld−/−Il18−/− mice were cultured for 24h (n=8 mice per group), and the IL-18 concentration in the supernatant was measured by ELISA and normalized to colon weight (***P<0.0001). c, mRNA from colonic mucosa of age- and sex-matched C. rodentium-infected wild-type, Cyld−/−, Nlrp6−/− and Cyld−/−Nlrp6−/− mice was isolated (n=8 mice per group), and the level of Il18 was quantified by real-time PCR (NSP=0.9748, NSP=0.2241, NSP=0.5841, left to right). d, Colon tissues from C. rodentium-infected wild-type, Cyld−/−, Nlrp6−/− and Cyld−/−Nlrp6−/− mice (n=8 mice per group) were cultured for 24h, and the IL-18 concentration in the supernatant was measured by ELISA and normalized to colon weight (***P=0.0001; NS, not significant). The statistics are given as mean ± s.d., with P values determined by Student’s t-test (two tail). The data are from one experiment representative of three independent experiments with similar results.