Fig. 2.

Minimal impact on sensitivity for reduced PCR DNA input for Mutation 1. Sensitivity to measure the AAF and background error through a dilution curve of a polymorphism (Mutation 1) using a 50 ng 0.01% to 50% AAF and data subsets with AAFs b less than 9% and c less than 0.08%. Reduction of DNA input to 25 ng with d 0.01% to 50% AAF and data subsets with AAFs e less than 9% and f less than 0.08%