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. 2021 Feb 12;12:128. doi: 10.1186/s13287-021-02186-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Increased levels of Crry expression and Akt activation in neurons in the brains of CHI mice receiving iNSCs pre-treated with CR2-Crry. a–c Representative staining for Crry⁺ (green, a), p-AKT⁺ (green, b), AKT⁺ (green, c), and NeuN⁺ (red) cells depicting the distribution of Crry⁺/NeuN⁺, p-AKT⁺/NeuN⁺, and AKT⁺/NeuN⁺ neurons in the injured cortex among the PBS (CHI mice receiving PBS), iNSC (CHI mice receiving iNSCs pre-treated with PBS), and iNSC (CR2-Crry) (CHI mice receiving iNSCs pre-treated with CR2-Crry) groups on day 14 post-CHI. Nuclei were counterstained with DAPI (blue). d Representative staining for NeuN⁺ (green) and TUNEL⁺ (red) cells depicting the distribution of NeuN⁺/TUNEL⁺ neurons in the injured cortex among the three groups at 14 days after CHI. Nuclei were counterstained with DAPI (blue). e–h Histograms indicating the ratio of Crry⁺/NeuN⁺ (e, the number of Crry and NeuN double-positive cells/the total number of DAPI-positive cells), p-AKT⁺/NeuN⁺ (f, the number of p-AKT and NeuN double-positive cells/the total number of DAPI-positive cells), and AKT⁺/NeuN⁺ (g, the number of AKT and NeuN double-positive cells/the total number of DAPI-positive cells), and NeuN⁺/TUNEL⁺ (h, the number of NeuN and TUNEL double-positive cells/the total number of DAPI-positive cells) cells in the injured cortex among the three groups on day 14 post-CHI (n = 6/group; one-way ANOVA, **P < 0.01, ***P < 0.001). Scale bar = 50 μm (5 μm)