Table 3.
Primer list used to characterize cIECs designed with PrimerBLAST
| Gene | Primer sequence (5′- > 3′) | Product length (bp) | Accession N. | |
|---|---|---|---|---|
| Epithelial markers | E-cadherin |
F: TGAAGACAGCCAAGGGCCTG R: CTGGCGGTGGAGAGTGTGAT |
109 | NM_001039258 |
| Villin |
F: GAACCTCTCGTGGCACCGC R: CTCATGTCCCTGCACCTCCC |
152 | XM_418521.5 | |
| Cytokeratin 18 |
F: CACAGATCCGGGAGAGCCTG R: CTCCACCGCGCTGTCATAGA |
110 | XM_025145666 | |
| Cytokeratin 20 |
F: GCGCGTTATAAAGGAGGAGCTG R: CGCTGATTTACGGGCCGAAC |
200 | NM_204749.2 | |
| Zonula occludens-1 |
F: TCTGCACAGTGAGGTTGGCT R: GGCTGTCCTGCATCGGTGT |
145 | XM_004934975 | |
| Occludin-1 |
F: TGCTTTTGCCCAAGCAGGAA R: TGTGGGAGAGGCACCAGTTG |
153 | NM_204417 | |
| Claudin-1 |
F: TCGGTGGTGGTCACTTCGTC R: CGCTGATTTACGGGCCGAAC |
113 | NM_001004768 | |
| Staminal markers | LGR5 |
F: TGGGCTCCACAGCCTAGAGA R: CCTACAAACGCACGCTCAGG |
144 | XM_425441.5 |
| SOX9 |
F: CCACCATGTCGGATGACTCC R: GGTGTTCTCTTGAGGTCGGG |
86 | NM_204281.1 | |
| CDXA |
F: CAGTGAGTGTCCCCCATGTC R: GGGACAGATGTCTGCAGGTC |
92 | NM_204676.2 | |
| CDXB |
F: ATCTGGTTCCAGAATCGCCG R: TGGTGGGAACAGGGAACTTG |
141 | NM_204614.1 | |
| Endothelial cell markers | CD34 |
F: TCTGCACAGTGAGGTTGGCT R: GGCTGTCCTGCATCGGTGT |
153 | XM_004934975 |
| CD45 |
F: TCCGACGCAGAGTGAATGCT R: AGCCCCTCCAACATAGCGTC |
118 | NM_204417.2 | |
| CD146 |
F: TCACAAAGCCAGAGGCACGTA R: AGCAGGAAAGGGTCGAAGGA |
196 | NM_001004768 | |
| CD90 |
F: GAACGTCTACCGGAACCGAG R: GTGTAGTCGTTGGTGGCCTT |
126 | NM_204381.2 | |
| CD73 |
F: GGGCACTCTGGCTTTACTGT R: GGAGGGAGGGGTTCCTGTAT |
111 | XM_004940396 | |
| CD105 |
F: GCGAAAATAGCAACAGCCCC R: GTACAGCCCTTCACCTCACG |
103 | NM_001080887 | |
| WNT and EGF pathways | AXIN2 |
F: ACCGAGACGTTGGAGAACGG R: ATCCGTCAGGGCATCACTGG |
138 | NM_204491.1 |
| c-MYC |
F: CTGGTCCTCAAGCGGTGTCA R: GACCCTGCCACTGTCCAACT |
114 | XM_015283089 | |
| LEF1 |
F: CCCACCCATCTCACATCCCC R: GTGAAAACCAGCCAAGAGGTGG |
141 | NM_205013.2 | |
| MAPK-1 |
F: TGTGACTTCGGACTGGCTCG R: AGGAGCCCTGTACCAACGTG |
93 | XM_015275131 | |
| Cyclin-D1 |
F: CGAGGTGGAGACCATCCGAC R: GAAGTAGGACACCGAGGGCG |
112 | NM_205381 | |
| Cyclin-E |
F: CGTTCCTCCAGGATCCCGAC R: CCAGGACAGCTGGTTTTCGT |
80 | NM_001031358 | |
| EGFR |
F: CCGCAGCTGGGTAGGCAC R: GCTCCTCACTCTGCACTGCT |
129 | NM_205497 | |
| Reference | RPLP0 |
F: TCACGGTAAAGAGGGGAGGTG R: CTTGCTCAGTCCCCAGCCTT |
143 | NM_205179 |