Table 4.

Antibodies used for immunofluorescence characterization

Reagent	Dilution	Product catalog number
Mouse anti-chicken cytokeratin 18	10 μg/mL	MA1–06326
Rabbit anti-chicken zonula occludens 1	10 μg/mL	61–7300
Rabbit anti-chicken pan-occludin	2 μg/mL	71–1500
Mouse anti-chicken vimentin	2 μg/mL	MA5–11883
Rabbit anti-villin	10 μg/mL	ab130751
Rabbit anti-MAPK1/ERK2	1:100	MA5–15134
Mouse ant-c-Myc	10 μg/mL	MA1–16637
Donkey anti-mouse secondary antibody, TRITC conjugated	4 μg/mL	A16016
Goat anti-rabbit secondary antibody, FITC conjugated	4 μg/mL	A27034

All antibodies were purchased from Invitrogen (Thermo Fisher Scientific, Waltham, Massachusetts, USA) except anti-villin that was purchased from Abcam plc (Cambridge, UK)