Figure 4:

Subtractive coordinate immunoreactivity pattern (SCIP) in cell-block section. Metastatic adenocarcinoma (cell-block sections of pleural fluid. History of triple-negative infiltrating duct carcinoma). Follow the neoplastic cells (arrows) (a) in serial sections to evaluated coordinate immunoreactivity pattern for at least two positive and two negative lineage-specific immunomarkers. The tumor cells (arrows) in dual color immunostained section are immunoreactive for BerEP4 (brown) and non-immunoreactive for vimentin (red) (b). They are also immunoreactive for cytokeratin 7 (c) with nuclear immunoreactivity for GATA3 (d). They are nonimmunoreactive for calretinin (a few adjacent reactive mesothelial cells are immunoreactive) (e) and cytokeratin 20 (f).