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. Author manuscript; available in PMC: 2022 Feb 9.
Published in final edited form as: Immunity. 2021 Jan 11;54(2):276–290.e5. doi: 10.1016/j.immuni.2020.12.004

Figure 6. ILC1s induce an antiviral state in the uninfected epithelium.

Figure 6

(A) Confocal images of a frozen tissue cross-section of uninfected labial mucosa of T-bet ZsGreen mice. Panel on the right lacks T-bet-ZsGreen to more easily visualize the IFN-γ staining (white). Scalebars = 20 μms. Results were repeated at least 3 times with 2–4 mice/group.

(B) As in (A) except in the uninfected labial mucosa of Ncr1-gfp+/+ mice. Scalebars = 10 μms.

(C) As in (B) except IFN-γ staining was performed using the XMG1.2 Ab. Scalebars = 10 μms.

(D) Confocal images of two different fields from a cross-section of the uninfected labial mucosa of a GREAT+/+ mouse. Scalebars = 50 μms (left) and 20 μms (right).

(E) Flow histograms of all ILCs from the labial mucosa of uninfected WT (red) or GREAT+/+ (light green) mice. Dark green histogram is gated on the small population of GREAT-YFPHI ILC1s.

(F) Geometric mean fluorescent intensity of GREAT-YFP in the populations shown in (E) (left panel). Right panel shows the percentage of GREAT-YFPHI ILC1s in B6 versus WT. Data are pooled from 2 experiments with 3 mice/group.

(G) qPCR array data of antiviral genes in the uninfected labial mucosa showing fold mRNA regulation of Rag2−/−Il2rg−/− mice compared to Rag1−/− mice. Results were pooled from 2 separate experiments with 3 mice/group.

(H) As in (F) but in uninfected Rag1−/− mice after treatment with IFN-γ-neutralizing Ab compared to untreated, uninfected Rag1−/− mice. Results were pooled from 2 separate experiments with 3 mice/group.

(I) Confocal images of a frozen tissue cross-section of the uninfected labial mucosa of a T-bet-ZsGreen mouse. Right panels omit DAPI signal to more easily visualize IRF7 staining (white). Boxed area is magnified in the image to the right. Scalebars = 100 μms (left and middle) and 20 μms (right).

Statistically significant differences in mRNA were determined using a Student’s t-test on the replicate ΔΔCT values for each gene in the control and treatment groups are shown above gene where applicable. Dots show individual mice. Confocal images were repeated at least 2 times with 2–4 mice/group, except for (D).

See also Figure S6.