Table 1.

Description of hemolytic activity Riemerella anatipestifer SX mutants

Mutants	Hemolytic zonesa	Description of encoded proteins	Locus tag in DSM15868b	Gene products
				Subcellular locationc	Putative conserved domain	Function group (COGs)e
M1	almost no	hypothetical protein	Riean_0373	Outer Membrane	–d	–f
M2, M25	almost no	hypothetical protein	Riean_0790	Outer membrane	CarboxypepD_reg	–
M3, M26	almost no	OmpA/MotB domain protein	Riean_1027	Outer membrane	OmpA_C_like, OmpA	COG2885 M
M4	almost no	TonB-dependent receptor plug	Riean_1561	Outer membrane	CirA, OMP_RagA_SusC	–
M5	reduced greatly	hypothetical protein	Riean_1374	Cytoplasmic membrane	–	–
M6	almost no	diacylglycerol kinase catalytic region	Riean_0288	Cytoplasmic	LCB5, DAGK_cat, DAGKC	COG1597 IR
M7, M28	almost no	TPR repeat protein	Riean_0317	Cytoplasmic	TPR_1	COG0790 T
M8	reduced greatly	adenylosuccinate lyase	Riean_0391	Cytoplasmic	PurB, ADSL_C, PRK08937	COG0015 F
M9	almost no	Pyruvate dehydrogenase (acetyl-transferring)	Riean_0589	Cytoplasmic	AcoB,Transket_pyr, Transketolase_C TPP_PYR_E1-PDHc-beta_like	COG0022 C
M10	almost no	Mg chelatase, subunit ChlI	Riean_1262	Cytoplasmic	YifB, Mg_chelatase, AAA	COG0606 O
M11	almost no	bacterial translation initiation factor 2	Riean_1302	Cytoplasmic	InfB, IF-2, IF2_Eif5B	COG0532 J
M12	almost no	YicC-like domain-containing protein	Riean_1527	Cytoplasmic	PRK11820, YicC, YicC_N	COG1561 S
M13	almost no	riboflavin biosynthesis protein RibD	Riean_1661	Cytoplasmic	RibD1, RibD_C	COG0117 H
M14	almost no	ATPase associated with various cellular activities AAA_3	Riean_1768	Cytoplasmic	AAA_3, AAA	COG0714 R
M15	reduced greatly	RelA/SpoT domain protein	Riean_1808	Cytoplasmic	NT_Rel-Spo_like, RelA_SpoT	COG2357FT
M16	almost no	RagB/SusD domain protein	Riean_0025	Unknown	SusD	-
M17	reduced greatly	alkyl hydroperoxide reductase/thiol specific antioxidant/Mal allergen	Riean_0256	Unknown	Thioredoxin_8, TrxA	COG0450 V
M18, M27, M29, M31	reduced greatly	hypothetical protein	Riean_0653	Unknown	–	–
M19	almost no	outer membrane transport energization protein ExbD1	Riean_0932	Unknown	ExbD, tolR	COG0848 U
M20	almost no	hypothetical protein	Riean_0968	Unknown	–	–
M21, M30	almost no	Zn-dependent aminopeptidase	Riean_1143	Unknown	M1_APN_5	COG2234 O
M22	reduced greatly	PSP1 domain protein	Riean_1185	Unknown	PSP1	COG1774 T
M23	almost no	Ferritin Dps family protein	Riean_1386	Unknown	Dps, Ferritin, PRK09448	COG0783 PV
M24	increased	homogentisate 12-dioxygenase	Riean_1546	Cytoplasmic	HmgA, PLN02658	COG3508 Q

^aThe sizes of hemolytic zone of the mutants on duck blood agar were compared to that of the wild strain SX. The mutants that exhibited almost no, very weak or increased hemolytic zones were examined

^bGenBank accession No.: CP002346

^cSubcellular locations were predicted by the PSORTb v.3.0 server [16]

^dNo conserved domain was predicted by Conserved Domain Database (CDD) [24]

^eCOG functional categories: (1) Information storage and processing (J: Translation, ribosomal structure and biogenesis); (2) Cellular processes.(M: Cell wall/membrane/envelope biogenesis; O: Post-translational modification, protein turnover, chaperones; T: Signal transduction mechanisms; V: Defense mechanisms; U: Intracellular trafficking,secretion,and vesicular transport); (3) Metabolism: (C:energy production and vonversion;E: Amino acid transport and metabolism; F: Nucleotide transport and metabolism; P: Inorganic ion transport and metabolism; I: lipid metabolism; G: Carbohydrate transport and metabolism; H. Coenzyme transport and metabolism; Q: secondary metabolites biosynthesis,transport and catabolism;). (4) Poorly characterized: (R: General function prediction only; S: function unknown)

^f – No related COG