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. 2021 Feb 12;19:44. doi: 10.1186/s12951-021-00791-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Evaluation of the cellular uptake of siRNA/peptide nanocomplexes using a fluorescence microscope. 200 nM Cy3-labeled siRNAs were delivered into 1.0 × 10⁵ HeLa cells via PC: Lipofectamine™ 2000, SPACE, R11, S-R7, S-R11, and S-R15 (20:1 N/P ratio) for 4 h. Nucleus and actin filaments were labeled using Hoechst 33342 (blue) and Phalloidin (green), respectively. The nanocomplex was observed in orange at 200× magnification (scale bar = 100 µm)