Table 1.
Strains and plasmids used in this study
| Plasmids and strains | Characteristics | Source/ Reference |
|---|---|---|
| Plasmids | ||
| pORI280 | RepA− gene replacement vector, constitutive lacZ, 5.3 kb, Emr | [97] |
| pORI280ADΔlmo1013 |
pORI280 containing 503-bp region on either side flanking lmo1013 deletion pORI280 including both upstream and downstream flanking regions (503 bp each) of lmo1013 deletion |
This study |
| pORI280ADΔlmo2229 |
pORI280 containing 503-bp region on either side flanking lmo2229 deletion pORI280 including both upstream and downstream flanking regions (503 bp each) of lmo2229 deletion |
This study |
| pVE6007 | Temperature-sensitive helper plasmid that provides RepA in trans, Cmr | [98] |
| E. coli strains | ||
| EC10B | DH10B derivative with Kanr and RepA integrated in the glgB gene | [99] |
| EC10B/pORI280ADΔlmo1013 | E. coli EC10B with pORI280ADΔlmo1013 | This study |
| EC10B/pORI280ADΔlmo2229 | E. coli EC10B with pORI280ADΔlmo2229 | This study |
| L. monocytogenes strains | ||
| EGDe | Serotype 1/2a | [99, 100] |
| EGDeΔlmo1013 | EGDe with the entire lmo1013 gene deleted | This study |
| EGDeΔlmo2229 | EGDe with the entire lmo2229 gene deleted | This study |