Table 2.

Primers used in the amplification of AB and CD fragments from L. monocytogenes EGDe genomic DNA

Primers	Sequence 5′- 3′	Amplimer obtained
lmo1013_AB_fwd	TCGAATTCGAAGCTTCTGCATAACACCAATAGTCGCCCCT	Upstream flanking fragment (AB) of lmo1013 coding region including the start codon (ATG)
lmo1013_AB_rev	AGCGAATTGGCGTCTTTTTACATTTTTTGGTCCACATCCT
lmo1013_CD_fwd	AGGATGTGGACCAAAAAATGTAAAAAGACGCCAATTCGCT	Downstream flanking fragment (CD) of the lmo1013 coding region including the stop codon (TAA)
lmo1013_CD_rev	ATGACGTCGACGCGTCTGCATAGTGCAGTTATTACGATTG
lmo2229_AB_fwd	TCGAATTCGAAGCTTCTGCATCAGGTGGCTCGATTGCAAA	Upstream flanking fragment (AB) of the lmo2229 coding region including the start codon (ATG)
lmo2229_AB_rev	GTAAAATGTGCTTTTAATTACATGTAACTCTCCTATCTTC
lmo2229_CD_fwd	GAAGATAGGAGAGTTACATGTAATTAAAAGCACATTTTAC	Downstream flanking fragment (CD) of the lmo2229 coding region including the stop codon (TAA)
lmo2229_CD_rev	ATGACGTCGACGCGTCTGCATTCACCATCTAAAGTAATTT