Fig. 2.

Low-dose DB increased cisplatin-sensitivity in CR-GC cells by inducing apoptotic and pyroptotic cell death. a, b cell proliferation was determined by CCK-8 assay, and c, d cell viability was measured by trypan blue staining assay. e Annexin V-FITC/PI double staining assay was performed to examine cell apoptosis ratio in CR-GC cells. Western Blot analysis was conducted to examine the expression levels of f, g pyroptosis associated signatures (NLRP3, ASC, IL-1β and IL-18) and h, i apoptosis associated proteins (cleaved Caspase-3 and Bax) in CR-GC cells. j, k The silencing vectors for NLRP3 were delivered into CR-GC cells. l Cell apoptosis was examined by Annexin V-FITC/PI double staining method. Each experiment repeated at least three times. *P < 0.05