Fig. 4.

Low-dose DB downregulated PD-L1 to inhibit CSCs properties in CR-GC cells. The CR-GC cells were administered with low-dose DB for 48 h, a spheroid formation abilities were evaluated by the spheroid formation assay, b, c the mRNA levels and d, e protein levels of SOX2, OCT4 and Nanog were determined by using the Real-Time qPCR and Western Blot analysis, respectively. f, g PD-L1 protein levels were determined by conducting Western Blot analysis. h, i The overexpression vectors for PD-L1 were delivered into CR-GC cells. The expression levels of SOX2, OCT4 and Nanog were examined at both j, k transcriptional and l, m translated levels. Each experiment repeated at least three times. *P < 0.05