Fig. 6.

Low-dose DB downregulated PD-L1 to trigger apoptotic and pyroptotic cell death in cisplatin treated CR-GC cells. Western Blot analysis was performed to determine a, b PD-L1 and NLRP3, and c, d pyroptosis associated biomarkers. e Annexin V-FITC/PI double staining method was used to examine cell apoptosis. f, g CCK-8 assay was used to measure cell proliferation, and h, i trypan blue staining assay was employed to determine cell viability. Western Blot analysis was employed to determine the expression levels of j, k proliferation associated proteins (Cyclin D1 and CDK2) and l, m apoptosis associated proteins (cleaved Caspase-3 and Bax) in mice tumor tissues. (Note: “Con” indicated “Control”, “Cis” suggested “Cisplatin”, “C + D” indicated “Cisplatin plus DB”, “C + D + P” indicated “Cisplatin plus DB plus PD-L1 overexpression”). Each experiment repeated at least three times. *P < 0.05