Figure 5.

Electrophysiological Alterations in hPSC-CMs Infected with SARS-CoV-2

(A and B) (A) Representative images of SARS-CoV-2-infected H7 hESC-CMs on MEA wells at 72 hpi. Scale bars, 50 μm. (B) Intracellular and extracellular viral particles in H7 hESC-CMs seeded and infected in the MEA plate. Intracellular viral particles are plotted on the right axis as viral RNA (percent of HPRT1); extracellular viral particles are plotted on the left axis as PFU/mL. Data are shown as mean ± SEM of eight wells. Differences versus mock control by two-way ANOVA with Sidak correction for multiple comparisons (^∗p < 0.05, ^∗∗∗p < 0.001).

(C) Representative propagation maps at 72 hpi. The axes illustrate the position of 16 total electrodes, with black dots indicating inactive ones. Electrical propagation starts in the blue area and moves toward the red area, according to the color-coded time interval.

(D) Representative recordings of spontaneous electrical activity of SARS-CoV-2-infected H7 hESC-CMs at 72 hpi.

(E and F) (E) Representative quantifications of electrophysiological properties from MEA analyses in SARS-CoV-2-infected H7 hESC-CMs. Mean ± SEM of eight wells. Statistical analyses of intra-experimental variability as for (B). (F) Representative field potential traces in SARS-CoV-2-infected H7 hESC-CMs at 72 hpi, and quantification of FPD corrected by beat rate in spontaneous and paced experiments. Mean ± SEM of eight and six wells for spontaneously beating and paced cells, respectively. Statistical analyses of intra-experimental variability as for (B) (^∗∗p < 0.01).