Table I.

Sequences of primers used and their real-time qRT-PCR programmes

Genes	Primer sequence (5’- 3’)	Real-time qRT-PCR programme#	Product size (bp)
HULC	F: ATCGTGGACATTTCAACCTC	D: 95°C for 25 sec	161
	R: GCTGTGCTTAGTTTATTGCC	A: 59°C for 25 sec
		E: 72°C for 25 sec
Bax	F: GCAAACTGGTGCTCAAGG	D: 95°C for 30 sec	236
	R: ACTCCCGCCACAAAGA	A: 63°C for 35 sec
		E: 72°C for 30 sec
Bcl-2	F: TGGGAAGTTTCAAATCAGC	D: 95°C for 25 sec	298
	R: GCATTCTTGGACGAGGG	A: 63°C for 30 sec
		E: 72°C for 30 sec
CDH1	F: AGTACAACGACCCAACCCAAG	D: 95°C for 30 sec	235
	R: GCAAGAATTCCTCCAAGAATCC	A: 57°C for 22 sec
		E: 72°C for 20 sec
VIM	F: CAGGCAAAGCAGGAGTCCA	D: 95°C for 30 sec	122
	R: AAGTTCTCTTCCATTTCACGCA	A: 59°C for 35 sec
		E: 72°C for 30 sec
MMP-9	F: CCGCTCACCTTCACTCGC	D: 95°C for 30 sec	174
	R: ACCACAACTCGTCATCGTC	A: 63°C for 35 sec
		E: 72°C for 30 sec
β-actin	F: AGAGCTACGAGCTGCCTGAC	D: 95°C for 30 sec	184
	R: GCAAGAATTCCTCCAAGAATCC	A: 59°C for 30 sec
		E: 72°C for 30 sec

^#real-time qRT-PCR cycling was started for all primers with initial denaturation at 95°C for 10 minutes. D, denaturation; A, primer annealing, E, extension; real-time qRT-PCR, real-time quantitative reverse transcription polymerase chain reaction; F, forward; R, reverse