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. Author manuscript; available in PMC: 2021 Feb 13.
Published in final edited form as: Mol Cancer Res. 2018 Jun 19;16(10):1530–1542. doi: 10.1158/1541-7786.MCR-18-0047

Figure 4: Aromatase constructs expressed in T47D cells alter aromatase activity.

Figure 4:

A, Time course of N-V5-tagged aromatase-WT construct expression in T47D cells. B, Transcript levels of V5-aromatase WT and mutant constructs and loading control beta actin in T47D cells after 24hrs. C, Expression of N-terminally tagged V5-aromatase constructs, endogenous aromatase (Total aromatase) and endogenous control Gapdh in T47D cells untreated or treated with androstenedione for 2 days (A4–2days). D, Estradiol levels of V5-aromatase WT and K108R/K108Q transfected cells treated with/without 10nM androstenedione for 2 days (A4–2days). E, Estradiol levels of V5-aromatase WT and K440R/K440Q transfected cells with/without 10nM androstenedione after 2 days (A4–2 days). Data is representive of three independent experiments carried out in triplicates with mean ± SEM *** p<0.0001, * p<0.05.