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. Author manuscript; available in PMC: 2021 Feb 13.
Published in final edited form as: Mol Cancer Res. 2018 Jun 19;16(10):1530–1542. doi: 10.1158/1541-7786.MCR-18-0047

Figure 6: K108/K440 mutations do not change the subcellular localization of aromatase.

Figure 6:

A, representative fluorescence images of N-V5-aromatase (magenta) in stably transfected HEK293 cells with empty vector (EV), N-V5-aromatase (N-V5-AROM), K108R mutant (N-V5-K108R), K108Q mutant (N-V5-K108Q), K440R mutant (N-V5-K440R) and K440Q mutant (N-V5-K440Q) proteins are shown. Merge of nuclear staining with DAPI (blue) and N-V5-aromatase (magenta) proteins is shown as DAPI/AROM for each of the mutant. B, Endoplasmic reticulum fractions (ERec extract) and total cell extract of HEK293 cells stably transfected with WT and mutant aromatase constructs showing V5-aromatase, calnexin and Gapdh endogenous control.