Figure 5.

β-Cell Bmal1 overexpression reverses β-cell functional failure in response to diet-induced obesity in male mice. A and B: Individual insulin concentration profiles (traces) and corresponding means ± SEMs sampled at 2-min intervals during islet perifusion performed at 4 mmol ⋅ L⁻¹ glucose (0–32 min), 16 mmol ⋅ L⁻¹ glucose (32–64 min), and back to 4 mmol ⋅ L⁻¹ glucose (64–96 min) in islets isolated from chow- and HFD-fed control (^+/+Ins2^rtTA/+) and β-Bmal1^OV (Bmal1-HA^tetO/+Ins2^rtTA/+) male mice. C: Mean basal (mean insulin concentration during 4 mmol ⋅ L⁻¹ glucose), first-phase (mean first peak insulin concertation at 16 mmol ⋅ L⁻¹ glucose), and second-phase (mean insulin concentration during 16 mmol ⋅ L⁻¹ glucose after the first peak) insulin responses during perifusions of islets from chow- and HFD-fed control (^+/+Ins2^rtTA/+) and β-Bmal1^OV (Bmal1-HA^tetO/+Ins2^rtTA/+) male mice. Data are mean ± SEM (n = 3 independent experimental perifusions per genotype/diet using isolated islets from n = 3 mice per genotype/diet). *P < 0.05. mM, mmol ⋅ L⁻¹.