Figure 1.

A significant reduction in β-cell proliferation and blood insulin concentrations of Adipoq^−/− dams. WT and Adipoq^−/− mice were cross-mated for avoidance of any fetal effect. Tissue samples were collected at G12.5 of fed dams. A: Blood insulin concentrations were determined by ELISA. B: We measured β-cell areas using immunochemistry-stained pancreatic sections. Four sections per pancreas were used. C and D: Pancreatic sections were probed by IF with anti-insulin (green) and anti-Ki67 antibody (red, indicated by arrowhead). A total of 8–10 islets was counted for positive cells of each pancreas. The average of the Ki67-positive percentage from each pancreas was calculated and used for statistical comparison (D). White scale bar, 50 μm. Data are presented as mean ± SEM.