Figure 4.

Adiponectin treatment increased PL expression in placental explants and JEG3 cells. Placental villi explants (A and B) and confluent JEG3 cells (C) were treated with adiponectin (2 μg/mL) for 6 h or overnight, respectively. The PL protein levels in the placental explants were measured by Western blotting (A). mRNA of CSH1 and CSH2 was determined by real-time PCR. The expression level of CSH2 is very low in JEG3 cells. Therefore, only CSH1 data of JEG3 cells are presented (C). Data are presented as mean ± SEM. AU, arbitrary units.