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. 2020 Oct 13;70(1):227–239. doi: 10.2337/db20-0375

Figure 8.

Figure 8

LncRNA MALAT1-Keap1 in retinal microvessels of diabetic mice and human donors with documented diabetic retinopathy. A–D: Retinal microvessels from diabetic mice (Diab) were analyzed for LncRNA MALAT1 expression (A) by qRT-PCR, LncRNA MALAT1 at the Keap1 promoter (B) was quantified employing ChIRP technique by Fluorescein dUTP-incorporated oligo (LncRNA MALAT1) immunoprecipitation, followed by quantification of Keap1 (C) and HO1 and Sod2 (D) gene transcripts by qRT-PCR using 18S rRNA as a housekeeping gene. Values obtained from age-matched normal mice (Nor) were considered as 1. E–H: Retinal microvessels from human donors with documented diabetic retinopathy (Diab Ret) were analyzed for LncRNA MALAT1 (qRT-PCR) (E), LncRNA MALAT1 at the Keap1 promoter (ChIRP) (F), and gene transcripts of Keap1 (G) and HO1 and Sod2 (H) by qRT-PCR, using β-actin as a housekeeping gene. Values from human donors without diabetes (Non-diab) were considered as 1. *P < 0.05 compared with normal mice, or to donors without diabetes. The values are represented as mean ± SD, and each measurement was made in duplicate in 7–9 mice per group or 6–7 human samples per group.