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. 2020 Nov 25;70(2):562–576. doi: 10.2337/db20-0660

Figure 2.

Figure 2

Knockdown of EGFR attenuated increased rubicon expression and promoted autophagic flux in cultured podocytes. A and B: siRNA knockdown of podocyte EGFR led to less activation of EGFR signaling and decreased SQSTM1, rubicon, and phosphorylated (p-)S6K expression. C and D: High glucose–induced beclin-1 inhibition and rubicon stimulation in podocytes were abolished by siEGFR. E and F: siRNA knockdown of podocyte EGFR increased LC3B abundance after exposure to high glucose compared with control siRNA, which promoted autophagosome production. *P < 0.05 and **P < 0.01 vs. siControl group with high glucose (HG), N = 3 independent repeats, one-way ANOVA with Bonferroni post hoc test. AU, arbitrary units.