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. 2020 Nov 25;70(2):562–576. doi: 10.2337/db20-0660

Figure 5.

Figure 5

Podocyte EGFR deficiency decreased renal fibrosis in glomerulus and tubulointerstitium in nos3−/− db/db mice. Both egfrf/f; nos3−/−; db/db (nos3−/−; db/db) mice and nphs2-Cre; egfrf/f; nos3−/−; db/db (EgfrpodKO; nos3−/−; db/db) mice were sacrificed at 20 weeks old. A: EGFR deletion in podocytes led to markedly decreased renal fibrosis in glomerulus and tubulointerstitium, as indicated by Sirius red staining. Original magnification: ×200. B: EGFR deletion in podocytes led to decreased renal mRNA levels of profibrotic and fibrotic components, including Col1a1 (collagen I), Col4a1 (collagen IV), and Tgfb1 (TGF-β1). **P < 0.01, n = 6 in each group. C–E: EGFR deletion in podocytes also decreased renal protein expression of col1a1, col4a1, acta2, and CTGF in both glomerulus and tubulointerstitium. *P < 0.05, n = 3 in nos3−/−; db/db group and n = 4 in egfrpodKO; nos3−/−; db/db group. Two-tailed unpaired Student t test.