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. 2020 Dec 1;70(2):577–588. doi: 10.2337/db20-0146

Figure 1.

Figure 1

AMPKα1 inhibition enhances exosome release from adipocytes and WAT. A: The exosomes internalized by adipocytes that were treated with AICAR (1 mmol/L) or CC (50 nmol/L) were counted using the EXOCET Kit. B: Samples with equal amounts of exosomes were analyzed for CD63 and CD81 with Western blotting. C and D: Densitometric analysis of the Western blots from B. E: Quantification of exosomes in the medium of 3T3L1-derived adipocytes that were transfected with siPrkaα1 or siPrkaα2. F: Samples with equal amounts of exosomes were analyzed for CD63 and CD81 by Western blotting. G and H: Densitometric analysis of the Western blots from F. I: Quantification of exosomes in WAT in WT, Prkaα1−/−, and Prkaα2−/− mice (n = 5). J: Samples with equal amounts of exosomes were analyzed for CD63 and CD81 by Western blotting. K and L: Densitometric analysis of the Western blots from J. *P < 0.05 vs. control (n = 3); #P < 0.05 vs. control group treated with AICAR treatment; $P < 0.05 vs. control group treated with siPrkaα1−/− or Prkaα1−/− group (n = 3).