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. 2020 Dec 1;70(2):577–588. doi: 10.2337/db20-0146

Figure 6.

Figure 6

AMPKα1 deficiency increases HFD-enhanced exosome shedding from WAT and exacerbates lipid accumulation in the liver. A: Quantification of serum exosomes (exo) in Prkaα1fl/fl, Prkaα1−/−, AdipoCre, and AlbCre mice fed an HFD for 3 months (n = 4 for each group). B: Western blot for CD63 in the exosomes from A. C: Quantification of exosomes in the supernatant of Prkaα1fl/fl, Prkaα1−/−, AdipoCre, and AlbCre WAT. D: Western blot for CD63 in the exosomes from C. E–M: Prkaα1fl/fl, Prkaα1−/−, AdipoCre, and AlbCre mice were fed an HFD for 3 months. E: Body weight. F: Increase in body weight. G: Liver weight. H: Ratio of liver weight to body weight. I: Serum cholesterol (CHO) levels. J: Serum triglyceride (TG) levels. K: Hepatic CHO levels. L: Hepatic TG levels. M: Representative images of Oil Red O and H&E (HE) staining of the liver tissues. *P < 0.05 vs. Prkaα1fl/fl mice; #P < 0.05 vs. Prkaα1−/− mice (n = 3); $P < 0.05 vs. AdipoCre mice (n = 3). AdipoCre, Prkaα1fl/fl:AdipoCre mice; AlbCre, Prkaα1fl/fl:AlbCre mice.