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. 2021 Feb 9;14:891–904. doi: 10.2147/OTT.S278233

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© 2021 Wu et al.

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miR-494-3p counteracted the anti-proliferative and pro-apoptotic effects of overexpressed WT1-AS. (A) CCK-8 assay was performed to evaluate the influence of miR-494-3p promotion on the anti-proliferative effects of overexpressed WT1-AS in A549 cells. (B and C) TUNEL staining was applied to assess the influence of miR-494-3p promotion on the pro-apoptotic effects of overexpressed WT1-AS in A549 cells. (D) Western blot assay was conducted for the detection of anti-apoptotic protein (Bcl-2) and pro-apoptotic proteins (Bax and cleaved caspase-3). *p<0.05, **p<0.01, ***p<0.001.