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a, Schematic of the pegFinder workflow for designing CRISPR prime editing pegRNAs. b, Criteria by which pegFinder identifies candidate pegRNA spacers that are suitable for the desired genomic edit. c, Overview of the factors considered by pegFinder when ranking and selecting candidate pegRNA spacers. d, Design principles by which pegFinder prioritizes PBS sequences and RT templates that compose the 3’ extension of pegRNAs.
