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. Author manuscript; available in PMC: 2021 Feb 14.
Published in final edited form as: Cell Rep. 2021 Jan 26;34(4):108690. doi: 10.1016/j.celrep.2021.108690

Figure 2. Reduced metabolic amplifying pathway in CDK2-iKO β cells.

Figure 2.

(A and C) Capacitance increases in response to 10 step depolarizations from −70 mV to 0 mV in (A) β cells (Con, n = 19; CDK2-iKO, n = 39) and (C) α cells (Con, n = 14; CDK2-iKO, n = 14) isolated from 4 Con and 3 CDK2-iKO mice.

(B and D) Averaged leak-subtracted calcium current (Ica) and influx (Qca) from β cells (B) and α cells (D), measured during a 15-ms step depolarization from −70 mV to 0 mV for each cell above.

(E) Ex vivo KCl-stimulated insulin secretion normalized to DNA content, measured in isolated islets from Con (n = 3) and CDK2-iKO (n = 4) mice, quantified by AUC.

Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by t test (B and D) or one-way ANOVA (E).