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. Author manuscript; available in PMC: 2021 Feb 14.
Published in final edited form as: Cell Rep. 2021 Jan 26;34(4):108690. doi: 10.1016/j.celrep.2021.108690

Figure 3. Islets with short-term CDK2 restriction have reduced Kir6.2 mRNA, resulting in reduced KATP channel activity.

Figure 3.

(A and C) Kcnj11 expression measured by qPCR in pancreatic islets treated with vehicle Con or CDK2i (n = 3 mice) (A) or islets isolated from Con and CDK2-iKO mice (n = 3 mice per genotype) (C).

(B and D) Measurements of KATP conductance in islets treated with vehicle Con or CDK2i (B) or CDK2-iKO islets (C). Left: representative current-voltage relationship collected during a voltage ramp, showing conductance (slope) changes in β cells measured in 10 mM glucose (10G) and after treatment with 200 μM diazoxide (DZ). Right: KATP conductance was reduced in CDK2i-treated (n = 7) relative to vehicle-treated Con (n = 11) β cells (B) and in CDK2-iKO β cells relative to Cons treated with Ad-Cre (n = 11 cells from 3 mice each genotype) (D).

Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by t test (A and C) or one-way ANOVA (B and D).