Figure 5.
AQmabAM protection against antibody-dependent cellular cytotoxicity (ADCC) produced by NMO patient sera and NK cells. A. ADCC induced by 1 h incubation of 1–2% NMO serum and NK cells as effector cells, with AQmabAM added prior to serum and NK cells. Cytotoxicity was measured using Alamar blue. B. AQmabAM concentration-dependent protection against cytotoxicity as in A (mean ± S.E.M., n=4) using NMO sera four different patients. C. IC50 for protection against ADCC by AQmab and AQmabAM with each point representing a different human NMO serum (mean ± S.E.M., n=4). D. Live/dead cell assay as done in B, with live cells stained green and dead cells stained red.