Table 1.
Composition of HS and CS from bat lung and human lung.
| HS TriS | HS NS6S | HS NS2S | HS NS | HS 2S6S | HS 6S | HS 2S | HS 0S | Total NS a | Total NAc b | Total sulfation c | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Male bat lung HS | 0.16 ± 0.10 % | 3.63 ± 1.41 % | 25.8 ± 3.19 % | 48.1 ± 2.94 % | 0.16 ± 0.16 % | 2.45 ± 1.06 % | 1.56 ± 1.24 % | 18.1 ± 6.82 % | 77.7 ± 6.47 % | 22.3th±5.11 % | 112 ± 15.1 % |
| Female bat lung HS | 0.34 ± 0.06 % | 3.02 ± 0.45 % | 23.8 ± 3.62 % | 47.3 ± 2.80 % | 0.03 ± 0.06 % | 3.53 ± 0.51 % | 1.15 ± 0.67 % | 20.8 ± 4.77 % | 74.5 ± 4.78 % | 25.5 ± 4.78 % | 107 ± 12.4 % |
| All bat lung HS | 0.25 ± 0.12 % | 3.32 ± 1.02 % | 24.8 ± 3.34 % | 47.7 ± 2.69 % | 0.09 ± 0.13 % | 2.99 ± 0.97 % | 1.35 ± 0.95 % | 19.4 ± 5.63 % | 76.1 ± 5.54 % | 23.9 ± 4.89 % | 109 ± 14.0 % |
| Human lung HS | 0.12 ± 0.21 % | 0.43 ± 0.55 % | 2.44 ± 2.37 % | 6.75 ± 3.82 % | 0.00 ± 0.00 % | 5.12 ± 0.97 % | 6.27 ± 1.11 % | 78.9 ± 7.74 % | 9.74 ± 6.81 % | 90.3 ± 6.81 % | 24.2 ± 12.4 % |
| CS TriS | CS 2S4S | CS 2S6S | CS 4S6S | CS 4S | CS 6S | CS 2S | CS 0S | / | / | Total sulfation d | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Male bat lung CS | 0.00 ± 0.00 % | 1.54 ± 2.37 % | 0.54 ± 1.08 % | 0.30 ± 0.59 % | 72.2 ± 0.68 % | 8.74 ± 1.22 % | 5.34 ± 3.81 % | 11.3 ± 4.41 % | / | / | 163 ± 13.9 % |
| Female bat lung CS | 0.00 ± 0.00 % | 3.53 ± 1.26 % | 3.44 ± 1.20 % | 0.21 ± 0.42 % | 66.7 ± 2.83 % | 12.7 ± 4.77 % | 4.28 ± 2.92 % | 9.15 ± 2.53 % | / | / | 164 ± 18.7 % |
| All bat lung CS | 0.00 ± 0.00 % | 2.54 ± 2.06 % | 1.99 ± 1.87 % | 0.25 ± 0.48 % | 69.4 ± 3.53 % | 10.7 ± 3.86 % | 4.81 ± 3.19 % | 10.2 ± 3.52 % | / | / | 164 ± 22.5 % |
| Human lung CS | 0.06 ± 0.04 % | 2.11 ± 0.58 % | 0.44 ± 0.08 % | 1.12 ± 0.52 % | 84.2 ± 1.76 % | 5.80 ± 1.82 % | 4.67 ± 0.12 % | 1.55 ± 0.49 % | / | / | 185 ± 7.41 % |
%S = 0×%0S + 1×%4S + 1×%6S + 1×%2S + 2×%2S4S + 2×%2S6S + 2×%4S6S + 3×%Tris. Values represent means ± standard deviations of 4 male bat lungs, 4 female bat lungs, and 6 male human lungs.
a
The total NS indicates all the HS disaccharides containing N-sulfo (NS) group.
b
The total NAc indicates all the HS disaccharides containing N-acetyl (NAc) group.
c
The total sulfation of HS (%S) is calculated by the following formula. %S = 0×%0S + 1×%NS + 1×%2S + 1×%6S + 2×%NS6S + 2×%NS2S + 2×%2S6S + 3×%Tris.
d
The total sulfation of CS (%S) is calculated by the following formula.